Theknoloji ea Ultrafiltration ke theknoloji e ncha le e phahameng ea karohano.E na le litšoaneleho tsa ts'ebetso e bonolo, phaello e phahameng ea moruo, ha ho na phetoho ea mekhahlelo, coefficient e kholo ea karohano, ho boloka matla, ts'ebetso e phahameng, ha ho na tšilafalo ea bobeli, ts'ebetso e tsoelang pele mocheso oa kamore joalo-joalo.Kajeno, mookameli Yang eo Beijing a ileng a botsa ka lisebelisoa tsa rona tsa ultrafiltration bakeng sa tlhoekiso ea protheine mme a buisana ka botlalo le theknoloji ea rona.Hona joale, mohlophisi oa sehlopha sa Shandong Bona o tla hlahisa ts'ebeliso ea ultrafiltration karohanong ea protheine le tlhoekiso.
1. Bakeng sa protheine desalination, dealcoholization le mahloriso
Lisebelisoa tsa bohlokoa ka ho fetisisa tsa ultrafiltration ho hloekisoeng ha liprotheine ke desalting le ho tsepamisa mohopolo.Mokhoa oa Ultrafiltration oa ho tlosa letsoai le ho tsepamisa mohopolo o khetholloa ke molumo o moholo oa batch, nako e khuts'oane ea ts'ebetso le ts'ebetso e phahameng ea ho hlaphoheloa ha protheine.Mokhoa oa setso oa steric exclusion chromatography ho tlosa lintho tse fapaneng ho tsoa ho liprotheine o nkeloe sebaka ke theknoloji ea sejoale-joale ea ultrafiltration, e fetohileng theknoloji e ka sehloohong ea protheine desalination, dealcoholization le concentration kajeno.Lilemong tsa morao tjena, theknoloji ea ultrafiltration e 'nile ea sebelisoa haholo ho tlosa letsoai le ho hlaphoheloa ha liprotheine tsa boleng bo phahameng ba phepo e nepahetseng ka har'a cheese whey le whey ea soya.Lactose le matsoai le likarolo tse ling tsa protheine, hammoho le litlhoko tsa sebele tsa ho phethahatsa ka katleho desalting, de-alcoholization le mahloriso a liprotheine.Ts'ebeliso ea theknoloji ea ultrafiltration e ka boela ea tsepamisa maikutlo a serospecies immunoglobulins ho fihlela tlhoko ea 'nete ea tlhahiso ea protheine.
2. Bakeng sa ho arohana ha protheine
Karohano ea protheine e bolela mokhoa oa ho arola karolo e 'ngoe le e 'ngoe ea protheine ho ea ka karolo ho latela phapang ea thepa ea 'mele le ea lik'hemik'hale (joalo ka boima ba molek'hule, ntlha ea isoelectric, hydrophobicity, joalo-joalo) ea motsoako ka mong oa protheine mokelikeli oa phepelo.Gel chromatography ke o mong oa mekhoa e sebelisoang haholo ho arola limolek'hule tsa bioloji (haholo-holo liprotheine).Ha ho bapisoa le chromatography ea setso, theknoloji ea karohano ea ultrafiltration e na le tebello e ntle ea ts'ebeliso ho karohano le tlhahiso ea indasteri ea liprotheine le li-enzyme tse nang le bohlokoa ba moruo ka lebaka la theko e tlase le ho holisoa habonolo.Lehe le lesoeu ke thepa e theko e tlaase ka ho fetisisa ea ho fumana lysozyme le ovalbumin.Haufinyane tjena, ultrafiltration e atisa ho sebelisoa ho arola ovalbumin le lysozyme ho tloha lehe le lesoeu.
3. Ho tlosoa ha Endotoxin
Ho tlosoa ha Endotoxin ke e 'ngoe ea mefuta ea mantlha ea ts'ebeliso ea theknoloji ea ultrafiltration tlhoekisong ea protheine.Mokhoa oa tlhahiso ea endotoxin o rarahane haholo.Ts'ebetsong ea ts'ebeliso e sebetsang, hobane protheine ea meriana e hlahisoang ke prokaryotic expression system e bonolo ho tsoakana le endotoxin e hlahisoang ke ho robeha ha lerako la baktheria, 'me endotoxin, e tsejoang hape e le pyrogen, ke mofuta oa lipopolysaccharide.Ka mor'a ho kena 'meleng oa motho, e ka baka feberu, tšitiso ea microcirculation, endotoxic shock le matšoao a mang.Bakeng sa ho sireletsa bophelo bo botle ba batho, hoa hlokahala ho sebelisa theknoloji ea ultrafiltration ka botlalo ho tlosa li-endotoxins.
Le hoja theknoloji ea ultrafiltration e sebelisoa haholo ho arola le ho hloekisa liprotheine, e boetse e na le meeli e itseng.Haeba boima ba limolek'hule tsa lihlahisoa tse peli tse lokelang ho arohana li ka tlase ho linako tse 5, li ke ke tsa aroloa ke ultrafiltration.Haeba boima ba molek'hule ba sehlahisoa bo le ka tlase ho 3kD, bo ke ke ba tsepamisoa ke ultrafiltration, hobane ultrafiltration hangata e etsoa ka bonyane ba boima ba molek'hule ba lera ho 1000 NWML.
Ka nts'etsopele e tsoelang pele ea indasteri ea bioengineering, litlhoko tse phahameng li behiloe pele bakeng sa karohano e tlase le theknoloji ea tlhoekiso.Mekhoa ea setso ea "vacuum concentration", "solvent extract", dialysis, centrifugation, precipitation le ho tloswa ha pyrogen ha e sa fumaneha ho fihlela litlhoko tsa tlhahiso.Theknoloji ea Ultrafiltration e tlameha ho sebelisoa haholo le ho feta hobane melemo ea eona karohanong ea protheine.
Nako ea poso: Apr-20-2022